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Ìý Ahead of its Time.

Fluorescently Labeled Actin Filaments.

Suzannah Miller, Biofrontiers Advanced Light Microscopy Core


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Biochemistry Cell Culture Facility: Past to Present

Around 1990, three Principal Investigators (PIs) from the Biochemistry Division in the Department of Chemistry and Biochemistry pooled their resources to create aÌýshared resource space. It began with a microscope, two incubators, and two biosafety cabinets, all donated by the three PIs involved. Together, theyÌýhired a technicianÌýand student helper to support the facility with media preparation, serum testing, biosafety cabinet maintenance, and training of new users.ÌýAs new faculty were hired,Ìýmore researchersÌýfound a need to use cell culture in their research and benefitted from the availability of the facility. When theÌýnumber of investigatorsÌýgrew toÌýapproximately ten labs, theÌýBiochemistry Cell Culture Facility was formalized.Ìý It currentlyÌýhas over 100 active users working in 19 different labs.

Growing Green

Because of the collaborative nature of the Biochemistry Cell Culture Facility, major milestones ​in the conservation of energyÌýand materialsÌýhave been made in conjunction with the efforts of ¶¶ÒõÂÃÐÐÉä Green Labs. A case study published in April 2018 revealed that the University saved over $800,000 in new construction costs (based on 2016 figures) in the building of the shared facility versus individual labs all having separate rooms. In addition,Ìýa yearly cost-avoidance of about $58,000 in maintenance, operation, utility, plug load and ventilation is realized by combining research into a shared space.

The facility participatesÌýin an ethanol recyclingÌýprogram where humidified, uncontaminated ethanol used byÌý¶¶ÒõÂÃÐÐÉä Boulder's Institute of Arctic and Alpine Research inÌýcold traps is diluted to 70% ethanol and used to decontaminate work surfaces and materials.Ìý The cost savings is around $1700/year.ÌýThe recycling of #2 and #4 plastic wrappers, in addition to paperboard, cardboard and office paper,Ìýhas diverted a significant waste stream from ending up in landfills.

Plastic recycling container in the laboratory.

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