抖阴旅行射

抖阴旅行射 Boulder has received a $1.1 million grant from the Bill & Melinda Gates Foundation to develop next-generation vaccines that require no refrigeration and defend against infectious diseases with just one shot.

If successful, those advancements could radically transform the difficult task of dispensing life-saving immunizations in developing countries鈥攁nd improve convenience in every part of the world.

Professor Bob Garcea of the and the has teamed up with Professors Ted Randolph and Al Weimer of the Department of Chemical and Biological Engineering in a unique collaboration that applies a wide range of skillsets and ideas to the pressing challenge of delivering vaccines to patients in developing countries. All three investigators work in the Jennie Smoly Caruthers Biotechnology Building (JSCBB) at 抖阴旅行射 Boulder, but their research areas have very different emphases.

鈥淚t鈥檚 really merging three different people with three different sets of expertise into one project,鈥� Garcea said.

In Garcea鈥檚 lab, located in the Jean L. and Jack C. Thompson Vaccine Development Laboratory of the JSCBB, investigators work on new vaccines such as those for human papillomavirus, a leading cause of cervical cancer that is particularly devastating to women in developing countries.

One corridor away, Randolph鈥檚 team, which focuses on creating stable dosage forms for therapeutic proteins and vaccines, developed a process for making vaccines thermostable, or resistant to damage from heat or cold. In this glassy powder state, the vaccine can be stored at temperatures as high as 120 degrees Fahrenheit for three to four months without losing efficacy, Randolph said.

The two began collaborating about two years ago and even formed a spinoff company, Vitravax Inc., which is seeing successful results in vaccine studies conducted in mice.

The Gates Foundation grant will take these innovations a step further by combining the thermostable vaccine powders with techniques developed in the Weimer lab that allow uniform nanoscopic protective layers of aluminum oxide to be applied to vaccine microparticles. This coating process, called atomic layer deposition, not only provides a nanometer-thick protective barrier for the vaccine particles but also helps trigger the body鈥檚 immune response.听听

The trio are now forming extended release, multilayer microparticulate vaccine dosage forms, composed of an inner core of stabilized vaccine coated with aluminum oxide layers and an outer layer of vaccine, all embedded in a glassy powder.听 When the formulation is injected, the outer layer provides an initial vaccine dose.听 Next, the aluminum oxide layer slowly dissolves, eventually releasing the inner core which acts as a second dose of vaccine. Patients receive their second or third 鈥渄ose鈥� without ever knowing it and without a return trip to the doctor.

Although each step of the process has worked independently, researchers cautioned that moving from small test batches in the lab to manufacturing millions of vaccines for public use is a challenging process that may not succeed quickly鈥攐r at all.

鈥淲e鈥檝e done many of the individual parts of this project,鈥� Randolph said. 鈥淣ow we鈥檝e got to put those pieces together and have it work.鈥�

Still, investigators say they are听optimistic about the collaboration, which might never have happened if not for their proximity on 抖阴旅行射 Boulder鈥檚 East Campus and the interdisciplinary mission of the BioFrontiers Institute, which seeks to drive innovation by combining researchers from different fields.

鈥淥ne of the hopes [of the BioFrontiers Institute]听is that investigators will, by their proximity, do new and interesting things,鈥� said Garcea, who is a member of the institute. 鈥淚n a sense, we鈥檝e fulfilled the mission. If the technology works, we鈥檝e really fulfilled the mission.鈥�